Intended Use
Singclean AFP ELISA Rapid Test Kit is intended for quantitative determination of the Alpha-fetoprotein (AFP) concentration in human serum.
Specification
48 tests / kit, 96 tests / kit
Testing procedure
Allow all reagents to reach room temperature before use:
1. Dilute wash buffer (20×) (1:19) with distilled water.
2. Label or mark the micro well strips to be used on the plate.
3. Pipet 50μL of AFP standards (0,5,20,50,200,500 ng/mL),AFP diluent (users as supplied), and serum samples into appropriate wells, mixed well, cover the plate and incubated at 37°C for 30min.
4. Aspirate and wash the wells 3 times with wash buffer. We recommend using an automatic ELISA plate washer for better consistency.
5. Add 100uL of anti-AFP-HRP conjugate reagent into each well. Cover the plate and incubate at 37°C for 30 min.
6. Aspirate and wash the wells 3 times with wash buffer as above.
7. Dispense 100μL HRP substrate solution into each well. Mix gently，cover the plate and incubate for 10 min at 37℃.
8. Add 50μL stop solution into each well and mixed gently.
9. Read optical density (OD) value at 450 nm in Elisa Analyzer within 30 minutes after addition of the stop solution.
10. The standard curve and regression equation were established by AFP standards
color intensity，then calculate the AFP concentration in samples (Correlation coefficient(r)≥0.9900).
Normal reference range
Normal reference range is ≤ 20ng/mL. Recommend the laboratories to establish their own normal reference range, the above data is only for reference.
Result Interpretation
If the sample concentration is higher than detection limit, it is feasible to dilute it with AFP diluent. Detection Concentration x Dilute Multiples = Original Concentration
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